Chimeric anti-EBOV GP

Cat #: 0201-021

Lot #:1401002

Immunogen: Venezuelan equine encephalitis virus replicons encoding ebolavirus (EBOV) glycoprotein (GP) was used to generate the original mouse monoclonal antibody.

Description: A murine / human chimeric IgG produced in N. benthamiana and is reactive to EBOV GP. The antibody detects GP in virus-like particles (VLP) and recombinant GP without the transmembrane region (rGPdTM).

Supplied: 100 µg is supplied at a concentration of 3.85 mg/mL. No preservative is added.

Purification: Antibody is purified using immobilized protein A.

Clonality: Murine variable, human constant of the IgG1 isotype.

Relevance: The antibody can be used for detection of EBOV GP. A mixture of all three anti-EBOV GP chimeric antibodies was protective against lethal challenge in a nonhuman primate study (Olinger et al. PNAS 2012, vol. 109, no. 44, 18030-18035).

Recommended Dilutions:

ELISA: Assay-dependent dilution

WB: Assay-dependent dilution. Internal QC demonstrates good detection when using antibody at 0.5 µg/mL.

Storage: 2-3 weeks +4oC, -20C long term

Cross Reactivity: No cross-reactivity was observed to Sudan ebolavirus (SUDV) or Marburg Virus (MARV) VLP or rGPdTM.

Chimeric anti-EBOV GP mAb (6D8)

Intended for research use only, not for human therapeutic or diagnostic applications.